Lipids play multiple roles in preserving neuronal function and synaptic plasticity, and polyunsaturated fatty acids (PUFAs) have been of particular interest in optimizing synaptic membrane organization and function. We developed a green-based methodology to prepare nanoliposomes (NL) from lecithin extracted from fish head by-products. These NL range between 100-120 nm in diameter, with an n-3/n-6 fatty acid ratio of 8.88. The high content of n-3 PUFA (46.3% of total fatty acid content) and docosahexanoic acid (26%) in these NL represented a means for enrichment of neuronal membranes potentially beneficial for neuronal growth and synaptogenesis. To test this, primary cultures of rat embryo cortical neurons were incubated with NL on day 3 post-culture for 24h, followed by immunoblots or immunofluorescence to evaluate NL effects on synaptogenesis, axonal growth and dendrite formation. Results revealed that NL-treated cells on day 4 displayed a level of neurite outgrowth and arborization similar to those of untreated cells on day 5 and 6, suggesting accelerated synapse formation and neuronal development in the presence of NL. We propose that fish-derived NL, by virtue of their n-3 PUFA profile and neurotrophic effects, represent a new innovative bioactive vector for developing preventive or curative treatments of neurodegenerative diseases.